Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells.

Ya-Nan Cao, Li-Juan Feng, Bang-Mao Wang, Kui Jiang, Shu Li et al.

Other Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association 2018 41 인용

<\/script>\n

`; }, get iframeSnippet() { const domain = 'gutcited.com'; const params = 'pmid\u003D29451186'; return ``; }, get activeSnippet() { return this.method === 'script' ? this.scriptSnippet : this.iframeSnippet; }, copySnippet() { navigator.clipboard.writeText(this.activeSnippet).then(() => { this.copied = true; setTimeout(() => { this.copied = false; }, 2000); }); } }" @keydown.escape.window="open = false" @click.outside="open = false">

Embed This Widget

Style

Widget powered by . Free, no account required.

Study Design

연구 유형: In Vitro
대상 집단: HT-29 and Caco-2 intestinal epithelial cells

중재: Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells. None
대조군: Control
일차 결과: SERT mRNA and protein expression
효과 방향: Positive
비뚤림 위험: Unclear

Abstract

BACKGROUND/AIMS: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. MATERIALS AND METHODS: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B. longum supernatants for 12 h and 24 h, respectively. SERT mRNA and proteins levels were detected by real-time polymerase chain reaction (real-time PCR) and Western-blotting. RESULTS: The mRNA levels of SERT in HT-29 and Caco-2 cells treated with different concentrations of L. acidophilus or B. longum supernatants for 12 h and 24 h, each, were higher than that in the control groups. In addition, the expression of the protein in both cells was also upregulated, which was approximately similar to that of the corresponding mRNA. CONCLUSIONS: L. acidophilus and B. longum supernatants can upregulate SERT mRNA and protein levels in intestinal epithelial cells.

요약

Investigation of the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein in intestinal epithelial cells found that they can upregulate SERT mRNA and protein levels in intestinal encapsulated cells.

Used In Evidence Reviews

B Bifidobacterium longum 관련 Irritable Bowel Syndrome (IBS)

B Lactobacillus acidophilus 관련 Irritable Bowel Syndrome (IBS)

Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells.

Embed This Widget

Study Design

Abstract

요약

Used In Evidence Reviews

Similar Papers

Lactobacillus acidophilus modulates intestinal pain and induces opioid and cannabinoid receptors.

Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome.

A meta-analysis of probiotic efficacy for gastrointestinal diseases.

Bacteriotherapy using fecal flora: toying with human motions.

Effects of Low FODMAP Diet on Symptoms, Fecal Microbiome, and Markers of Inflammation in Patients With Quiescent Inflammatory Bowel Disease in a Randomized Trial.

A double-blind, randomized, placebo-controlled trial of Lactobacillus helveticus and Bifidobacterium longum for the symptoms of depression.