**Figure 2.** Fig 1. Workflow and DNA microarray analysis. Workflow in the acquisition of an IBS genetic “fingerprint” from patient sample to transcriptomic analysis. Differentially expressed genes from mucosal tissue biopsy samples in the sigmoid colon of patients diagnosed with irritable bowel syndrome (IBS) and non-IBS patients (control). The heat map (adapted from Dussik et al.) displayed differential expression of 858 genetic features between the two experimental groups (p-value < 0.05) [68]. Pronounced clusters of similar color and intensity are also visible with blue indicative of individual biomarkers with lower expression levels and red illustrating higher levels of expression.

Mô tả

Workflow and DNA microarray analysis. Workflow in the acquisition of an IBS genetic “fingerprint” from patient sample to transcriptomic analysis.

More Figures from This Paper

Figure 3

Gene expression fold change in IBS patients relative to control patients using RT-qPCR testing. In total, 29 genes of interest were evaluated with only 23 being reported here (only those genes with at least two independent RT-qPCR plate replicates are included).

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Figure 4

IBS-Associated gene regulation by 1,25D. The mRNA levels of each gene were determined using quantitative real-time polymerase chain reaction (RTqPCR) for a total of three to six replicates.

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Figure 2

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Source Paper

Identification of putative transcriptomic biomarkers in irritable bowel syndrome (IBS): Differential gene expression and regulation of TPH1 and SERT by …

PloS one (2022)

PMID: 36264926

DOI: 10.1371/journal.pone.0275683

Cite This Figure

![Figure 2: Workflow and DNA microarray analysis. Workflow in the acquisition of an IBS genetic “fingerprint” from patient sample to transcriptomic analysis.](https://pdfs.citedhealth.com/figures/36264926/368.png)

> Source: Aleksandra Grozić et al. "Identification of putative transcriptomic biomarkers in irritable bowel syndrome." *PloS one*, 2022. PMID: [36264926](https://pubmed.ncbi.nlm.nih.gov/36264926/)

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  <img src="https://pdfs.citedhealth.com/figures/36264926/368.png" alt="Workflow and DNA microarray analysis. Workflow in the acquisition of an IBS genetic “fingerprint” from patient sample to transcriptomic analysis." />
  <figcaption>Figure 2. Workflow and DNA microarray analysis. Workflow in the acquisition of an IBS genetic “fingerprint” from patient sample to transcriptomic analysis.<br>  Source: Aleksandra Grozić et al. "Identification of putative transcriptomic biomarkers in irritable bowel syndrome." <em>PloS one</em>, 2022. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/36264926/">36264926</a></figcaption>
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