Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells.

Ya-Nan Cao, Li-Juan Feng, Bang-Mao Wang, Kui Jiang, Shu Li et al.

Other Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association 2018 41 件の引用

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Study Design

研究タイプ: In Vitro
対象集団: HT-29 and Caco-2 intestinal epithelial cells

介入: Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells. None
比較対照: Control
主要アウトカム: SERT mRNA and protein expression
効果の方向: Positive
バイアスリスク: Unclear

Abstract

BACKGROUND/AIMS: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. MATERIALS AND METHODS: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B. longum supernatants for 12 h and 24 h, respectively. SERT mRNA and proteins levels were detected by real-time polymerase chain reaction (real-time PCR) and Western-blotting. RESULTS: The mRNA levels of SERT in HT-29 and Caco-2 cells treated with different concentrations of L. acidophilus or B. longum supernatants for 12 h and 24 h, each, were higher than that in the control groups. In addition, the expression of the protein in both cells was also upregulated, which was approximately similar to that of the corresponding mRNA. CONCLUSIONS: L. acidophilus and B. longum supernatants can upregulate SERT mRNA and protein levels in intestinal epithelial cells.

要約

Investigation of the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein in intestinal epithelial cells found that they can upregulate SERT mRNA and protein levels in intestinal encapsulated cells.

Used In Evidence Reviews

B Bifidobacterium longum ： Irritable Bowel Syndrome (IBS)

B Lactobacillus acidophilus ： Irritable Bowel Syndrome (IBS)

Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells.

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Study Design

Abstract

要約

Used In Evidence Reviews

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